Using FRET to measure the angle at which a protein bends DNA
نویسندگان
چکیده
منابع مشابه
Single-molecule FRET measures bends and kinks in DNA.
We present advances in the use of single-molecule FRET measurements with flexibly linked dyes to derive full 3D structures of DNA constructs based on absolute distances. The resolution obtained by this single-molecule approach harbours the potential to study in detail also protein- or damage-induced DNA bending. If one is to generate a geometric structural model, distances between fixed positio...
متن کاملRight angle bends in light pipes: analysis.
An expression for the fraction of incident radiation transmitted by a right angle bend in a rectangular light pipe is derived. This expression is used to show that, in a planar light pipe structure with right angle bends, light attenuation is due only to reflection losses at the walls if the launching angle is 45 degrees .
متن کاملHow a short double-stranded DNA bends.
A recent experiment using fluorescence microscopy showed that double-stranded DNA fragments shorter than 100 base pairs loop with the probabilities higher by the factor of 10(2)-10(6) than predicted by the worm-like chain (WLC) model [R. Vafabakhsh and T. Ha, Science 337, 1101(2012)]. Furthermore, the looping probabilities were found to be nearly independent of the loop size. The results signif...
متن کاملA Guide to Fluorescent Protein FRET Pairs
Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most commonly used as both donor and acceptor fluorophores in FRET biosensors, especially since FPs are genetically encodable and live-cell compati...
متن کاملStudying Z-DNA and B- to Z-DNA transitions using a cytosine analogue FRET-pair
Herein, we report on the use of a tricyclic cytosine FRET pair, incorporated into DNA with different base pair separations, to study Z-DNA and B-Z DNA junctions. With its position inside the DNA structure, the FRET pair responds to a B- to Z-DNA transition with a distinct change in FRET efficiency for each donor/acceptor configuration allowing reliable structural probing. Moreover, we show how ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemistry and Molecular Biology Education
سال: 2008
ISSN: 1470-8175,1539-3429
DOI: 10.1002/bmb.20202